Ph Reports

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Unit 15 P5

PH report
Hypothesis: the aim of this experiment was to see the effects that different levels of PH values has upon amylase enzyme activity.
Apparatus:
* Stirring rod * Dimple tray * Five pipettes * Syringe * Starch solution * Amylase * Iodine * Buffer of PH4, 7 and 10 * Test tube holder * Stopwatch
Procedure: the first thing to do would be to ensure that you have all of the equipment needs for the experiment. The first step is to get a test tube and place it within the test tube holder, once you’ve done that you get a pipette using the pipette take some of the iodine and then very carefully put to drops of the iodine solution into the test tube. After that gets another pipette using that one to very gently to store 2 mL of the amylase enzyme solution, then very carefully put to drops of the amylase solution into the test tube. Once you’ve done that you get a third pipette and use that one to store 1 mL of the PH4 buffer, you then very carefully put the 1 mL of the pH4 buffer into the test tube, the second to last thing that is now added is 2 mL of starch. Once all of these chemicals have been put into one test tube it is then gently stirred, your lab partner will at the same time put 2 drops of iodine into 4 wells of the dimple tray, labelled 4 wells as 10 seconds, 20 seconds, 30 seconds and 40 seconds, using a stopwatch keep an eye on the time and wants 10 seconds of past use a syringe get a few drops of your amylase solution and put it into the well that is labelled 10 seconds, do the same thing once 20 seconds have gone by the one that is labelled 20 seconds, as well as the one that labelled 30 seconds and 40 seconds. Once you have done take note of the results, write down if there has been a colour change or if it remains the same thing, repeat the experiment 2 more times in the same manner except the…...

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