Mung Bean Respiration Lab

In: Science

Submitted By aspendrup14
Words 2265
Pages 10
The effect of glucose on the growth of mung beans as measured in percentages of glucose mixed in water
Annie Spendrup

I. Purpose

The purpose of this lab was to examine the effect of a glucose-water mixture on the growth and development of mung beans. Different percentages of glucose (0%, 1%, 2%, 3%, and 4%) in 40 millilitres of distilled water were used to water the mung beans.

The hypothesis is that seeds watered with a glucose water solution will grow faster and taller than the ones not watered with glucose mixed into the water because the glucose will give extra nutrients for the seeds and will provide extra glucose which will help then grow taller in a shorter amount of time.

II. Variables

Independent variable

The independent variable is how much 10% glucose solution is added to 40 ml of distilled water in order to create 1%, 2%, 3%, and 4% glucose solutions to use to water the mung beans.

Dependent variable

The dependent variable is how quickly it takes for the beans to sprout both roots and sprout. The number of days until the roots and stems sprout will be recorded, as well as how long the sprouts are.

Controlled variables
What needs to be controlled? Why does it need to be controlled / How would it impact the results? How will it be controlled?
Health of the beans when planted If the seeds weren’t in good health throughout the experiment (included in the beginning) the beans would either not grow or would not grow at all. This would skew the data as the data would depend on how long it took for the seeds to take root and grow. The seeds will be selected based on how they look. They will need to be a bright green, oval in shape, and have a creamy white hilum.
Amount of water given to the plants If each set of beans had differing amounts of water, the seeds with less water might not have evenly distributed water for all the…...

Similar Documents

Ib Biology Respiration Lab Paper

...Design Lab: Respiration The Effect of Heavy-Duty Exercise on the respiration of Homo Sapiens (Humans) Research Question: What is the effect of heavy-duty exercise (cardiovascular and other activities) on the respiration of humans? Background Information: In this experiment, trials will be conducted consisting of humans run up a set of stairs up and down 3 times as fast as they possibly can in order to put pressure on oxygen need to increase respiration needs. This will test the effect of a set exercise on the respiratory needs of a human. After this, they will have to do a set of 10 push-ups and 15 jumping jacks. If 10 push-ups are not possible, a substitute of 15 curl-ups should be administered instead. Hypothesis: When exercise is complete, the respiratory needs of one human should be significantly higher than it was previous to the exercise. Independent Variable: Human fitness level, exercise set Dependent Variable: How fast the human goes Controlled Variable: Temperature, time for exercise, amount of oxygen in air Materials: * Piece of paper for general observations as well as data recording * 4-6 humans * Timer (ipod, clock, something) * Water (if needed for human) Procedure: 1. Use the stairs next to the Biology classroom, select one human to go first and rotate turns until all humans have gone or until necessary data is gathered 2. Record the rate of exhales of the human (normal breathing) for 30 seconds and any other......

Words: 354 - Pages: 2

Effect of Environmental Stress on Growth of Mung Bean

...prominent aspect of growth and development of Mungbean is that the plant is sensitive to drought stresses, as a consequence of which growth and developments go significantly below potential. The evidence for the existence of moisture sensitive phases (when yield is decreased more by drought) in Mungbean is weak especially when Mungbean cultivated in rain fed condition. Mungbean cultivation has to undergo significant drought period in dry region of Bangladesh which is the major constrain of growth and pod filling. Therefore, the present experiment was undertaken to evaluate the effect of drought stress on the morph-physiological attributes of Mungbean and to find out optimum stages of irrigation for increased growth of Mungbean (cv. BINA mung 5) resulting maximum yield. In the arid and semi-arid regions, water deficit is the main factor that limits crops performance. Limitation of water source, irregular annual rainfall during growth season and lack of sources management cause severe decreasing in crops yield at these regions (Eack, 1996). Therefore, drought stress during growth season is an important problem that need to attention (khodabandeh, 2005). Using crops with short-term growth is one of the procedures to drought tolerance in dry regions. Mungbean is belongs to fabaceae family that currently is grown in different parts of world and it have large role in nutrition at developing countries (Dhingraetal 1991). Due to short-term growth, nitrogen fixation capability,......

Words: 1090 - Pages: 5

Cellular Respiration Lab

...Cellular Respiration My hypothesis for the germinated pea experiment was that the level of aerobic respiration would increase as the temperature would increase. I also predicted that there would be an upper temperature limit. The experiment proved part of my hypothesis correct and part of it incorrect. I was correct in predicting that the rate would increase as the temperature increase, but I was incorrect in hypothesizing that there would be an upper limit. My hypothesis for the larvae experiment was that the level of aerobic respiration would increase as the temperature increased and there would be an upper temperature limit. My hypothesis was proved to be correct, for the rate of respiration continued to increase until the upper limit was hit and the rate started to decline. There were no unexpected results from our group, but I noticed that group six had some unexpected data in the class larvae table. The only explanation I can make out of it is that the group made a math error. I do not have any suggestions to make this experiment better. Other factors that can affect the rate of cellular respiration are amount of available nutrients, because this allows more energy to be produced from the cell with an increase in amount of nutrients. Another factor is the state of the cell, such as the difference between working and dormant cells or the difference between plant and animal cells. From our data, we noticed that the temperature had an......

Words: 1061 - Pages: 5

Metabolism and Oxidative Cellular Respiration

...III: Oxidative Cellular Respiration Introduction Oxidative cellular respiration is composed of series metabolic processes that convert broken down food molecules into usable energy in the form of adenosine tri-phosphate (ATP). The process follows oxidation (catabolic) and reduction (anabolic) pathways. Processes involved are glycolysis, Krebs or tricarboxylic acid (TCA) cycle, and the electron transport chain. One step in the TCA cycle is the enzyme-catalyzed conversion of succinate to fumarate in a redox reaction. In intact cells succinate loses hydrogen ions and electrons to FAD to form fumarate. This step in the TCA cycle will be used to study the rate of cellular respiration under different conditions. (Patriquin, M. Rand, T. 2012). Since DPIP is a reducing dye it will absorb the hydrogen ions and electrons from the redox reaction of the TCA cycle between succinate and fumarate producing a discoloration of the dye. The discoloration is measured in percent transmittance of light over 30minutes at 5 minute intervals. The change in dye color is the associated with cellular respiration activity, and will be used to record the cellular respiration rate in mitochondria isolated from pulverized lima beans (Phaseolus lunatus) and subsequent effects of different substrate concentration, pH, and metabolic inhibitors . If the difference of light percent transmission produced by (DPIP) can be recorded over time associated with the cellular respiration rate then the......

Words: 1801 - Pages: 8

Cellular Respiration

...Cellular Respiration Lab Report Purpose The purpose of this lab was to find out which substance, molasses, sucrose, or the items of choice. This experiment was done to help the students understand which substance the yeast could perform cellular respiration in and why it could depending on it’s structure and bonds. From this experiment students should be able to better understand cellular respiration and why it occurs, like how it happens and how the organism benefits from cellular respiration. Also it should be understood what substances cellular respiration occurs on more easily and which substances take longer, and why that is because of what they are composed of. Hypotheses For this experiment there were two substances to be brought in and experimented with, substances that were tried were pure honey and water. 1). 50 ml of pure honey will produce 10 cm of carbon dioxide. 2). 25 ml of pure honey diluted with 25 ml of water will produce 7 cm of carbon dioxide. Introduction There are two processes to remove energy, that is aerobic and anaerobic. Anaerobic is the process of fermentation and requires no oxygen. Aerobic is cellular respiration and requires oxygen. Going further into aerobic is the process of cellular respiration is the process which energy is removed in the form of ATP, this process requires oxygen and a food source. All Oxygen using organisms perform cellular respiration. There are 3 major steps to cellular respiration: 1). Glycolysis- It...

Words: 611 - Pages: 3

Microbial Quality of Mung Bean (Vigna Radiata, L. Wilczek)

...INTRODUCTION Mung bean (Vigna radiata L. Wilczek) sprouts can be considered one of the most common vegetables consumed in the Philippines due to availability and nutritional value (Del Rosario, 2003 and FNRI, 1997). One cup of raw mung bean sprouts contains 48 calories, 6.5 grams of protein, 1.5 grams of fat, and 5.6 grams of carbohydrate. One cup of cooked bean sprouts contains 48 calories, 6.6 grams of protein, 1.8 grams of fat, and 4.6 grams of carbohydrate. In addition, sprouts are a good source of minerals and vitamins, particularly vitamins B1, B2, and C (http://ianrpubs.unl.edu/horticulture). Mung bean sprouts are low in calories and exceptionally high in potassium. A nutritious sprouted mung bean offers the same amount of vitamin A as a lemon, the niacin of a banana, the thiamine of an avocado, the riboflavin of a dried apple, the carbohydrate content of a melon and the ascorbic acid of a loganberry (http://www.specialtyproduce.com). The sprouting process encourages the rapid growth of microorganisms that reach very high numbers in the finished product (Fu et al., 2000). Seed sprouting provides an excellent environment for the growth of many types of organisms. The release of nutrients from the sprouting seeds, the moisture resulting from the irrigation process, the aerobic conditions, pH and temperatures favorable to mesophiles all contribute to the rapid expansion of microbial population, including food pathogens, during sprouting. Population as high......

Words: 6506 - Pages: 27

Lab Report: Cellular Respiration

...Cellular Respiration Ben Gipson BIO 1400 Exercise 6.1 Alcoholic fermentation is a metabolic pathway used primarily by yeast and some bacteria. In fermentation, glucose is broken down into ethyl alcohol (ethanol) and carbon dioxide. In the process, some of the energy that had been stored in the glucose bonds is used to form high energy bonds in ATP. Name some factors that affect enzyme activity. Temperature, pH, enzyme concentration, substrate concentration Materials: yeast, test tubes, beakers, rubber stoppers, wax pencil, water, corn syrup Procedure: 1. You will need to have three fermentation setups, so you should have six large test tubes, three pieces of plastic tubing that have been inserted into rubber stoppers, and three beakers (400 or 600 mL). Using a wax pencil, label three of the test tubes 1, 2, and 3 and set them aside. Assemble the setups one at a time following steps 2-7. 2. Fill a tub or sink with hot water. 3. Insert the end of the plastic tubing into one of the test tubes. This tube will be the CO2 collection tube. Submerge the collection tube and plastic tubing in the tub of hot water. 4. Submerge the beaker. Place the collection tube in the beaker in an inverted position. 5. Bring the beaker out of the water. One end of the plastic tubing should still be inserted in the collection tube. Hold up the other end of the tubing (the one with the rubber stopper on it) so that the water won’t be siphoned out. Setting up the CO2......

Words: 1260 - Pages: 6

Mung Bean Peeling Machine Operation Problems

...As a professional mung bean peeling machine manufacturer, we can also provide considerate solutions for your mung bean peeling machine operation attentions. Now we will introduce the operating attentions before mung bean peeling machine starting. 1.Connect the 380V power supply before mung bean peeling machine operation, then start the motor, and check whether the revolving direction keeps unanimous with the indicating arrow. If they are not unanimous, we can exchange the two splices at will, then they will become unanimous with the indicating arrow. 2. The beans that will be peeled should not be too dry, if not, they will be easily broken. The moisture content should keep about 9%, or the broken rate will increase. In this case, we can damp the beans with water first. In winter, it needs about 4 hours for dampening and two hours in spring. Then the beans can be processed by the mung bean peeling machine. This is quite important in MUNG BEAN PEELING MACHINE operation attentions. 3. Before processing, we should separate the beans by different species and granules. Then we use suitable size of screens, which can improve production efficiency and reduce broken rate. 4. After trial run of the mung bean peeling machine, if each part of the machine runs normally and without any noise, then we can begin the production. As a professional mung bean peeling machine manufacturer, we can also provide considerate solutions for your mung bean peeling machine operation......

Words: 444 - Pages: 2

Lab Report of Respiration

...Title: To investigate the effect of temperature on the rate of respiration. Introduction: In this experiment, we are going to investigate how the rate of respiration is affected by changes of temperature. Hypothesis: I think that the rate of respiration increases as the temperature increases; on the contrary, the rate of respiration decreases as temperature decreases. The reason is that as temperature increases until a certain point, acetyl-CoA works most efficiently, so that the amount of O2 and Co2 will increase as well. For instance, body temperature maintains constantly around 37℃. At this temperature, human beings can work more efficient than other extreme high or extreme low temperature. Experimental Design: Independent variable: Different temperatures (Inside & Outside) Dependent variable: The rate of respiration (Change in O2 & Change in Co2) Controls: * Timer * Pressure * Type + Number of worms * Volume of container * The initial amount of O2 and Co2 Materials: * 8 worms * 2 O2 sensors * 2 Co2 sensors * 2 Biochampers (250 ml) * 2 Thermometers * 2 Timers * 2 Lab Quest Minis * 2 Laptops Risk Assessment: Procedure: Put 4 worms in a biochamper inside the classroom. Set up O2 sensor and Co2 sensor in the biochamper. Set up O2 sensor, Co2 sensor and thermometer on the Lab Quest Mini, and then on the laptop. Wait for 2-3 minutes for Co2 inside to be even. Start the timer for......

Words: 668 - Pages: 3

Priming Affect Crop Stand of Mung Bean

...PRIMING AFFECT CROP STAND OF MUNG BEAN Ahmad Khan, Shad Khan Khalil*, Shakirullah Khan** and Ashfaq Afzal * Department of Agronomy, NWFP Agricultural University, Peshawar- Pakistan. ** Department of Extension Education and Commutation, NWFP Agricultural University, Peshawar- Pakistan. ABSTRACT Seed priming had increased germination of several plants particularly vegetables. The effect of PEG 8000 concentrations levels i.e. 100, 200, 300 g liter-1 water, water soaked and control (seed not treated) on germination m-2 and plant population hectare-1 at harvest of Mung bean varieties NM-92 and NM-98 seeds treated for 6 and 12 hours and then dried for a whole day in open air at room temperature, were studied at Agricultural Research Farm, NWFP Agricultural University, Peshawar during 2003. Emergence m-2 was significantly enhanced by 8% for treated seed compared to control but has non-significant improvement over water soaked. Emergence was increased from 56% (control) to 69% for seed treated 300 g PEG liter-1 water. Longer treatment duration (12 hours) significantly increased emergence m-2 by 1% compared to 6 hours treatment. Though non-significant result was found for plant at harvest yet increment in PEG concentrations had resulted in more number of plants at harvest for primed seed compared to non-primed seed. In conclusion PEG 8000 treated seeds showed better performance than both water soaking and control seeds. INTRODUCTION Mung bean (Vigna radiata......

Words: 2528 - Pages: 11

Exporting Beans

...Contents Executive Summary 2 A-1 Trading Company 3 Global production of beans that our company export 4 Importing Countries 5 Exporting Countries 6 Production of Myanmar 6 Export of Myanmar 7 Vision of the A-1 Trading Company 7 Mission of the A-1 Trading Company 7 Cross-cultural business between Myanmar and India 8 Information about India 9 Absolute advantage of the company 10 Addressing international trade to expand our company 11 Attracting FDI to fund and expand our company 12 Taking advantage on International Opportunities 13 Benefits to consumers 13 Strategies 14 Corporate Strategy 14 Business Strategy 14 Distribution Channel 15 Markets and Segments 16 Customer Segmentation 17 Customer Value Proposition 17 Conclusion 19 Executive Summary The purpose of this assignment is to develop a business strategy for our company, A1 Company Limited. Our company export beans and pulses to India and planning to attract FDI on strategic assets of company to produce value added products made of bean. In this report includes business relationship between Myanmar and India, the advantages we currently have and opportunities for entering other international markets. Also, our marketing plan of targeting customer segments, our company value to customers and our plans to help our suppliers with sophisticated technologies which will be a great help in improving the quality of our products. Finally, our future plan for producing value added......

Words: 4537 - Pages: 19

Lab Predator Prey Interactions: Bean Simulation

...------------------------------------------------- Lab # 1 ------------------------------------------------- Predator Prey Interactions: Bean simulation Enviornmental Science November 1, 2014 Objective: To keep record of the effects that the predator (coyote) and prey (mice) have on the population of each other over time. Introduction: Interactions between predators and their prey are important in 1) determining the population of both predators and prey and 2) determining and maintaining the structure of a community. Many factors enter into the ultimate outcome of predator-prey interactions. Using a simulation of predator-prey interaction including mice and coyote will be conducted to determine population over time in their habitat. Materials: * Shallow plate (the field) * Beans (the mice) * Spoons (the coyotes) Procedure: 1. Shake the plate to randomly distribute the mice. 2. Swipe the spoon through the field and capture mice. 3. Record data Mice Minimum mice per generation = 10 Maximum mice per generation = 100 Mice double every generation OR hit 100 Capture less than 5 mice and coyote dies Capture 5 or more and coyote survives Coyote 5 or more survive 6-9 mice = 1 baby 10-14 mice = 2 babies 15 or more = 3 babies Minimum coyote = 1 Maximum coyote = 17 Results Quantitative Results ...

Words: 656 - Pages: 3

Photosynthesis Cellular Respiration Lab

...Name _________________________________ Date _____________ Period ______ Lab: Investigating Photosynthesis and Cellular Respiration Background: All organisms are dependent on a healthy carbon dioxide-oxygen balance. Photosynthesis and cellular respiration are key processes in maintaining this balance. Plants, through the process of photosynthesis, use energy absorbed from sunlight, water, and carbon dioxide to produce sugars and oxygen. Animals and plants, through the process of cellular respiration, use oxygen and sugars to produce carbon dioxide, water, and the energy needed to maintain life. Purpose: To determine how carbon dioxide cycles through a biological system by performing the “Carbon Transfer Through Snails and Elodea” lab activity. Procedure: 1. Read through the background information and purpose. Create your own question for what you are investigating in this experiment. a. Problem (in question form): How are photosynthesis and cellular respiration related? 2. Obtain 8 test tubes of equal size/volume and number the test tubes 1-8. Fill each test tube with approximately 30 mL of deionized water. 3. Add enough bromothymol blue (BTB) indicator to solution to each test tube to change the water to a green color (about 3 mL). 4. What is Bromothymol Blue? Why is the BTB in the test tube green (Hint: recall the teacher demonstration)? Bromothymol blue is an indicator that changes color when carbon dioxide is present. Green......

Words: 1222 - Pages: 5

Bean Beetles Competition Lab

...Bean Beatle Competition Lab RESULTS: In the two control petri dish that consisted of 20 beans with eggs previously on them, the females laid two to three eggs. In the two control petri dish that consisted of 20 beans without eggs previously on them, the females laid to 2 to 6 eggs. The average number of eggs laid on the eggs that already had eggs on them was 1.25 eggs and the standard error was 0.5522. The average number of eggs laid on the eggs that did not already have eggs on them was 6.91667 and the standard error was 0.9728 (Figure 2). A comparative t-test was performed and from it the t-value, degrees of freedom, and p-value were determined. The t-value, for two-tailed distribution, was 2.20099, the degrees of freedom was 11, and the p-value was 0.00033 (Figure 1). DISCUSSION: Our hypothesis was accepted because the female bean beetles preferred to lay eggs on the beans without eggs rather than the beans with eggs already on them; therefore, the null hypothesis was rejected, because the p-value, 0.00033, was less than 0.05. This research is important because it tells us more about the ovipositional preferences of the female bean beetles. Larvae can’t move from the egg which is deposited, so the bean determined the future food resources that is available for their offspring, which would affect the the influence of the growth or reproduction; therefore, minimizing competition of resources between the offspring. It also important to know how if......

Words: 547 - Pages: 3

Toxicity to Mung Bean

...with mungbeans for: 3 controlled; 3 for first concentration (1ml); 3 for the second concentration (5ml) and 3 for third concentration (9ml). 6. Designate each different concentration to different areas in a plot. Record it. 7. Start adding the different concentration to the designated containers. 8. Repeat it at the same time everyday for five days. Observe changes and record. RESULTS: Day 1 Controlled 1ml 5ml 9ml Day 2 Controlled 1ml 5ml 9ml Day 3 Controlled 1ml 5ml 9ml Day 4 Controlled 1ml 5ml 9ml Day 5 Controlled 1ml 5ml 9ml INDEX Length of Mung beans | | Controlled | T1 | T2 | T3 | Day 1 | 0.5 | 0.5 | 0 | 0 | Day 2 | 2 | 1 | 1 | 0.75 | Day 3 | 3 | 1.75 | 1.5 | 1 | Day 4 | 4.5 | 3 | 2 | 1.25 | Day 5 | 8 | 6.58 | 4 | 2 | | Number of Mung beans | Controlled | 10 | T1 (1mL) | 10 | T2 (5mL) | 8 | T3 (9mL) | 6 | DISCUSSION: Mungbean plants with different trial and replicate number were distributed from different area of the given plot. From the 12 experiments, each 6 was divided into 3 sets. Set 1 was placed where there is abundant in sunlight, set 2 was placed on the sunlit areas and set 3 was placed where there is no much sunlight. It represents every day’s differences of the mungbean plants. It sums up the data of the experiment that the height and number of mungbean plants that grew varies from the concentrations. In the controlled data, plants were normally......

Words: 822 - Pages: 4